ID
Name
Class
Organism
Assay Summary
1
kalata B1
Cyclotide
Viola odorata Oldenlandia affinis Viola tricolor Hybanthus enneaspermus Viola baoshanensis Viola yedoensis Viola philippica Viola sumatrana Miq
CyO2, CyO13, kalata B1, and varv peptide A exhibited dose-dependent cytotoxicity in MTT assays with IC50 values of 2.15-7.93 uM against human brain astrocytoma cells (U-87 MG) and human bone marrow derived neuroblastoma cells (SH-SY5Y). CyO2 and varv peptide A also sensitized SH-SY5Y and U-87 MG cells to temozolomide (TMZ) which is the only effective chemotherapeutic drug used to treat glioblastoma multiforme (GBM). [...]
21
cycloviolacin O2
Cyclotide
Viola odorata Hybanthus enneaspermus Viola baoshanensis Viola biflora Viola philippica Viola uliginosa Viola arcuata Viola austrosinensis Viola anagae
CyO2, CyO13, kalata B1, and varv peptide A exhibited dose-dependent cytotoxicity in MTT assays with IC50 values of 2.15-7.93 uM against human brain astrocytoma cells (U-87 MG) and human bone marrow derived neuroblastoma cells (SH-SY5Y). CyO2 and varv peptide A also sensitized SH-SY5Y and U-87 MG cells to temozolomide (TMZ) which is the only effective chemotherapeutic drug used to treat glioblastoma multiforme (GBM). [...] Studies using calcein assay and HeLa cells showed that at 10 uM, the increase in calcein leakage is very rapid. [...] Psyle A, psyle C, psyle E and cycloviolacin O2 shown to possess cytotoxic activity in cancer cells. [...] Glutamic acid mutant had reduced activity [...] Cytotoxicity of cyO2, cyO9, hyen D and kB5 and their mutants on HeLa cells. [...] Cycloviolacin O2 showed antitumor effects are minor or absent at tolerable (sublethal) doses, and cycloviolacin O2 has a
very abrupt in vivo toxicity profile, with lethality after
single injection at 2 mg/kg, but no signs of discomfort to
the animals at 1.5 mg/kg. Repeated dosing of 1 mg/kg gave a local-inflammatory reaction at the site of injection after 2–3 days; lower doses were without complications. [...] Cell viability and morphology studies on the human lymphoma cell line U-937 GTB showed that cells exposed to cycloviolacin O2 displayed disintegrated cell membranes within 5 min. [...] All three cyclotides, varv A, varv F, and cycloviolacin O2, exhibited strong cytotoxic activities, which varied in a dose-dependent manner. [...]
27
cycloviolacin O8
Cyclotide
Viola odorata Viola baoshanensis Viola adunca Viola uliginosa
Cycloviolacin O8 showed micromolar anticancer activity against PC-3 prostate, MDA-MB-231 breast, and OVCAR-3 ovarian cancer cell lines and antifungal activity against the agricultural pathogenFusarium graminearum. [...]
34
cycloviolacin O9
Cyclotide
Viola odorata Viola biflora
Cytotoxicity of cyO2, cyO9, hyen D and kB5 and their mutants on HeLa cells. [...]
36
vitri A
Cyclotide
Viola odorata Viola tricolor Viola biflora Psychotria leptothyrsa
Vitri A, varv A and varv E shown to possess cytotoxic activity in cancer cells. [...]
37
kalata S
Cyclotide
Viola odorata Oldenlandia affinis Viola tricolor Viola arvensis Viola baoshanensis Viola yedoensis Viola biflora Viola philippica Viola ignobilis Viola uliginosa Viola sumatrana Miq Viola arcuata Palicourea sessilis Viola betonicifolia Viola anagae
CyO2, CyO13, kalata B1, and varv peptide A exhibited dose-dependent cytotoxicity in MTT assays with IC50 values of 2.15-7.93 uM against human brain astrocytoma cells (U-87 MG) and human bone marrow derived neuroblastoma cells (SH-SY5Y). CyO2 and varv peptide A also sensitized SH-SY5Y and U-87 MG cells to temozolomide (TMZ) which is the only effective chemotherapeutic drug used to treat glioblastoma multiforme (GBM). [...] All three cyclotides, varv A, varv F, and cycloviolacin O2, exhibited strong cytotoxic activities, which varied in a dose-dependent manner. [...] Vitri A, varv A and varv E shown to possess cytotoxic activity in cancer cells. [...]
38
cycloviolacin O12
Cyclotide
Viola tricolor Viola arvensis Viola baoshanensis Viola yedoensis Viola tianshanica Viola abyssinica Viola philippica Viola arcuata
Vitri A, varv A and varv E shown to possess cytotoxic activity in cancer cells. [...]
52
cycloviolacin O4
Cyclotide
Viola odorata Viola tricolor Viola baoshanensis Pombalia calceolaria
Cytotoxic activity against human cancer cell lines of anpy A, anpy B, anpy C, cyO4 and cyO17 [...]
59
kalata B5
Cyclotide
Oldenlandia affinis
Cytotoxicity of cyO2, cyO9, hyen D and kB5 and their mutants on HeLa cells. [...]
64
varv peptide F
Cyclotide
Viola tricolor Viola arvensis
All three cyclotides, varv A, varv F, and cycloviolacin O2, exhibited strong cytotoxic activities, which varied in a dose-dependent manner. [...]
170
cycloviolacin O13
Cyclotide
Viola odorata Hybanthus enneaspermus Viola baoshanensis Viola uliginosa Viola arcuata Viola austrosinensis
CyO2, CyO13, kalata B1, and varv peptide A exhibited dose-dependent cytotoxicity in MTT assays with IC50 values of 2.15-7.93 uM against human brain astrocytoma cells (U-87 MG) and human bone marrow derived neuroblastoma cells (SH-SY5Y). CyO2 and varv peptide A also sensitized SH-SY5Y and U-87 MG cells to temozolomide (TMZ) which is the only effective chemotherapeutic drug used to treat glioblastoma multiforme (GBM). [...]
177
cycloviolacin O17
Cyclotide
Viola odorata
Cytotoxic activity against human cancer cell lines of anpy A, anpy B, anpy C, cyO4 and cyO17 [...]
236
[Glu(Me)]cyO2
Cyclotide
Synthetic
Glutamic acid mutant had reduced activity [...]
237
[Lys(Ac)]2cyO2
Cyclotide
Synthetic
Glutamic acid mutant had reduced activity [...]
238
[Arg(CHD)]cyO2
Cyclotide
Synthetic
Glutamic acid mutant had reduced activity [...]
239
([Lys(Ac)]2[Arg(CHD)])cyO2
Cyclotide
Synthetic
Glutamic acid mutant had reduced activity [...]
355
vibi D
Cyclotide
Viola biflora
vibi D, E, G and H were analysed using fluorometric microculture cytotoxicity assay using a lymphoma cell line. The IC50 values ranged between 0.96 and 5.0 uM for vibi E, G and H, and vibi D was not cytotoxic at 30 uM. [...]
356
vibi E
Cyclotide
Viola biflora
vibi D, E, G and H were analysed using fluorometric microculture cytotoxicity assay using a lymphoma cell line. The IC50 values ranged between 0.96 and 5.0 uM for vibi E, G and H, and vibi D was not cytotoxic at 30 uM. [...]
358
vibi G
Cyclotide
Viola tricolor Viola biflora Psychotria leptothyrsa
vibi D, E, G and H were analysed using fluorometric microculture cytotoxicity assay using a lymphoma cell line. The IC50 values ranged between 0.96 and 5.0 uM for vibi E, G and H, and vibi D was not cytotoxic at 30 uM. [...]
359
vibi H
Cyclotide
Viola biflora Psychotria leptothyrsa
vibi D, E, G and H were analysed using fluorometric microculture cytotoxicity assay using a lymphoma cell line. The IC50 values ranged between 0.96 and 5.0 uM for vibi E, G and H, and vibi D was not cytotoxic at 30 uM. [...]
552
psyle A
Cyclotide
Psychotria leptothyrsa
Psyle A, psyle C, psyle E and cycloviolacin O2 shown to possess cytotoxic activity in cancer cells. [...] Nonclonogenic fluorometric microculture cytotoxicity assay was performed on psyle A, C and E using U-937 GTB human lymphoma tumor cell line. [...]
554
psyle C
Cyclotide
Psychotria leptothyrsa
Psyle A, psyle C, psyle E and cycloviolacin O2 shown to possess cytotoxic activity in cancer cells. [...] Nonclonogenic fluorometric microculture cytotoxicity assay was performed on psyle A, C and E using U-937 GTB human lymphoma tumor cell line. [...]
556
psyle E
Cyclotide
Psychotria leptothyrsa
Nonclonogenic fluorometric microculture cytotoxicity assay was performed on psyle A, C and E using U-937 GTB human lymphoma tumor cell line. [...] Psyle A, psyle C, psyle E and cycloviolacin O2 shown to possess cytotoxic activity in cancer cells. [...]
703
cyclolinopeptide A
Orbitide
Linum usitatissimum (flax)
cyclolinopeptide A and B exhibited apoptosis induction on SGC-7901 cells. [...]
704
cyclolinopeptide B
Orbitide
Linum usitatissimum (flax)
cyclolinopeptide A and B exhibited apoptosis induction on SGC-7901 cells. [...]
1139
hyen D
Cyclotide
Hybanthus enneaspermus
Cytotoxicity of cyO2, cyO9, hyen D and kB5 and their mutants on HeLa cells. [...]
1217
DC1
Cyclotide
Hedyotis diffusa
DC1-3 demonstrated potent cytotoxicity in prostate cancer cells and DC3 had a lowest IC50 value. [...]
1218
DC2
Cyclotide
Hedyotis diffusa
DC1-3 demonstrated potent cytotoxicity in prostate cancer cells and DC3 had a lowest IC50 value. [...]
1220
DC3
Cyclotide
Hedyotis diffusa
DC1-3 demonstrated potent cytotoxicity in prostate cancer cells and DC3 had a lowest IC50 value. [...]
1308
Rivi1
Cyclotide
Rinorea virgata
Rivi1-4 showed cytotoxic activities against MDA-MB-231 (breast cancer), MM96L (melanoma), HT-29 (colon cancer), human umbilical vein endothelia cells (HUVECs) and HFF-1 (human foreskin fibroblast) cells. Rivil1 was the least toxic Rivi peptides, Rivi 2−4 had only slightly higher IC50 values than cycloviolacin O2 and showed minimal selectivity between the cell lines. Rivi1−3 did not show hemolytic activity. [...]
1309
Rivi2
Cyclotide
Rinorea virgata
Rivi1-4 showed cytotoxic activities against MDA-MB-231 (breast cancer), MM96L (melanoma), HT-29 (colon cancer), human umbilical vein endothelia cells (HUVECs) and HFF-1 (human foreskin fibroblast) cells. Rivil1 was the least toxic Rivi peptides, Rivi 2−4 had only slightly higher IC50 values than cycloviolacin O2 and showed minimal selectivity between the cell lines. Rivi1−3 did not show hemolytic activity. [...]
1310
Rivi3
Cyclotide
Rinorea virgata
Rivi1-4 showed cytotoxic activities against MDA-MB-231 (breast cancer), MM96L (melanoma), HT-29 (colon cancer), human umbilical vein endothelia cells (HUVECs) and HFF-1 (human foreskin fibroblast) cells. Rivil1 was the least toxic Rivi peptides, Rivi 2−4 had only slightly higher IC50 values than cycloviolacin O2 and showed minimal selectivity between the cell lines. Rivi1−3 did not show hemolytic activity. [...]
1311
Rivi4
Cyclotide
Rinorea virgata
Rivi1-4 showed cytotoxic activities against MDA-MB-231 (breast cancer), MM96L (melanoma), HT-29 (colon cancer), human umbilical vein endothelia cells (HUVECs) and HFF-1 (human foreskin fibroblast) cells. Rivil1 was the least toxic Rivi peptides, Rivi 2−4 had only slightly higher IC50 values than cycloviolacin O2 and showed minimal selectivity between the cell lines. Rivi1−3 did not show hemolytic activity. [...]
1313
Parigidin-br3
Cyclotide
Palicourea rigida
Parigidin-br3 showed cytotoxic activity against MCF-7 (breast cancer) and CACO2 (colorectal adenocarcinoma) cells, with IC50 values of ∼2.5 μM and less than 10% hemolytic activity. [...]
1321
HB5
Cyclotide
Hedyotis biflora
HB5-9 showed significant cytotoxicity on cancer cell lines BxPC3, Capan2, MOH1 and PANC1. HB7 inhibited the cell
migration and invasion of Capan2 cells and could significantly inhibit the tumour weight and size. [...]
1322
HB6
Cyclotide
Hedyotis biflora
HB5-9 showed significant cytotoxicity on cancer cell lines BxPC3, Capan2, MOH1 and PANC1. HB7 inhibited the cell
migration and invasion of Capan2 cells and could significantly inhibit the tumour weight and size. [...]
1323
HB7
Cyclotide
Hedyotis biflora
HB5-9 showed significant cytotoxicity on cancer cell lines BxPC3, Capan2, MOH1 and PANC1. HB7 inhibited the cell
migration and invasion of Capan2 cells and could significantly inhibit the tumour weight and size. [...]
1324
HB8
Cyclotide
Hedyotis biflora
HB5-9 showed significant cytotoxicity on cancer cell lines BxPC3, Capan2, MOH1 and PANC1. HB7 inhibited the cell
migration and invasion of Capan2 cells and could significantly inhibit the tumour weight and size. [...]
1325
HB9
Cyclotide
Hedyotis biflora
HB5-9 showed significant cytotoxicity on cancer cell lines BxPC3, Capan2, MOH1 and PANC1. HB7 inhibited the cell
migration and invasion of Capan2 cells and could significantly inhibit the tumour weight and size. [...]
1935
[I11G]cyO2
Cyclotide
Synthetic
Cytotoxicity of cyO2, cyO9, hyen D and kB5 and their mutants on HeLa cells. [...]
1936
[I11L]cyO2
Cyclotide
Synthetic
Cytotoxicity of cyO2, cyO9, hyen D and kB5 and their mutants on HeLa cells. [...]
1937
[I11G]cyO9
Cyclotide
Synthetic
Cytotoxicity of cyO2, cyO9, hyen D and kB5 and their mutants on HeLa cells. [...]
1938
[I11L]cyO9
Cyclotide
Synthetic
Cytotoxicity of cyO2, cyO9, hyen D and kB5 and their mutants on HeLa cells. [...]
1939
[I11G]hyen D
Cyclotide
Synthetic
Cytotoxicity of cyO2, cyO9, hyen D and kB5 and their mutants on HeLa cells. [...]
1940
[I11L]hyen D
Cyclotide
Synthetic
Cytotoxicity of the hyen D variants. [...] Cytotoxicity of cyO2, cyO9, hyen D and kB5 and their mutants on HeLa cells. [...]
1941
[I11G]kB5
Cyclotide
Synthetic
Cytotoxicity of cyO2, cyO9, hyen D and kB5 and their mutants on HeLa cells. [...]
1942
[I11L]kB5
Cyclotide
Synthetic
Cytotoxicity of cyO2, cyO9, hyen D and kB5 and their mutants on HeLa cells. [...]
1944
[G1A, I11L]hyen D
Cyclotide
Synthetic
Cytotoxicity of the hyen D variants. [...]
1945
[F2A, I11L]hyen D
Cyclotide
Synthetic
Cytotoxicity of the hyen D variants. [...]
1946
[G5A, I11L]hyen D
Cyclotide
Synthetic
Cytotoxicity of the hyen D variants. [...]
1947
[S7A, I11L]hyen D
Cyclotide
Synthetic
Cytotoxicity of the hyen D variants. [...]
1948
[V9A, I11L]hyen D
Cyclotide
Synthetic
Cytotoxicity of the hyen D variants. [...]
1949
[Y10A, I11L]hyen D
Cyclotide
Synthetic
Cytotoxicity of the hyen D variants. [...]
1950
[I11A]hyen D
Cyclotide
Synthetic
Cytotoxicity of the hyen D variants. [...]
1951
[I11L, F14A]hyen D
Cyclotide
Synthetic
Cytotoxicity of the hyen D variants. [...]
1952
[I11L, I18A]hyen D
Cyclotide
Synthetic
Cytotoxicity of the hyen D variants. [...]
1953
[I11L, S21A]hyen D
Cyclotide
Synthetic
Cytotoxicity of the hyen D variants. [...]
1954
[I11L, K23A]hyen D
Cyclotide
Synthetic
Cytotoxicity of the hyen D variants. [...]
1955
[I11L, S24A]hyen D
Cyclotide
Synthetic
Cytotoxicity of the hyen D variants. [...]
1956
[I11L, K25A]hyen D
Cyclotide
Synthetic
Cytotoxicity of the hyen D variants. [...]
1957
[I11L, V26A]hyen D
Cyclotide
Synthetic
Cytotoxicity of the hyen D variants. [...]
1958
[I11L, Y28A]hyen D
Cyclotide
Synthetic
Cytotoxicity of the hyen D variants. [...]
1959
[I11L, K29A]hyen D
Cyclotide
Synthetic
Cytotoxicity of the hyen D variants. [...]
1960
[I11L, N30A]hyen D
Cyclotide
Synthetic
Cytotoxicity of the hyen D variants. [...]
1961
anpy A
Cyclotide
Anchietea pyrifolia
Cytotoxic activity against human cancer cell lines of anpy A, anpy B, anpy C, cyO4 and cyO17 [...]
1962
anpy B
Cyclotide
Anchietea pyrifolia
Cytotoxic activity against human cancer cell lines of anpy A, anpy B, anpy C, cyO4 and cyO17 [...]
1963
anpy C
Cyclotide
Anchietea pyrifolia
Cytotoxic activity against human cancer cell lines of anpy A, anpy B, anpy C, cyO4 and cyO17 [...]