[T20A]kalata B1(469) Protein Card

General Information
Name [T20A]kalata B1
Sequence GLPVCGETCVGGTCNTPGCACSWPVCTRN
Class Cyclotide
Average Mass 2862.25
Monoisotopic Mass 2860.13
m/z M+H 2861.14
ProteinType Mutant
Parent kalata B1
Organism Synthetic
Notes same as cycloviolacin B7
Cyclic Yes

Assay
Insecticidal
Substitution of certain residues of kalata B1 to alanine result in loss of insecticidal activity [...]Simonsen SM et al. (2008) J Biol Chem 283:9805-9813
Hemolytic
Substitution of certain residues of kalata B1 to alanine result in loss of hemolytic activity. Residues in loops 1 and 3 along with a few other isolated residues strongly affect activity. [...]Simonsen SM et al. (2008) J Biol Chem 283:9805-9813
Nematocidal
The cyclotides caused a concentration-dependent response in the LDA with the two nematode species. [...]Colgrave ML et al. (2008) Biochemistry 47:5581-5589

References
Simonsen SM, Sando L, Rosengren KJ, Wang CK, Colgrave ML, Daly NL, Craik DJ (2008) Alanine scanning mutagenesis of the prototypic cyclotide reveals a cluster of residues essential for bioactivity J Biol Chem 283:9805-9813

Cross-references
Nucleic acids
Structure
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