| Insecticidal | | Substitution of certain residues of kalata B1 to alanine result in loss of insecticidal activity [...] | Simonsen SM et al. (2008) J Biol Chem 283:9805-9813 |
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| Hemolytic | | Substitution of certain residues of kalata B1 to alanine result in loss of hemolytic activity. Residues in loops 1 and 3 along with a few other isolated residues strongly affect activity. [...] | Simonsen SM et al. (2008) J Biol Chem 283:9805-9813 |
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| Metal binding assay | | In the presence of Mn2+ kalata B1, T16A and T27A showed specific broadening of a defined set of amide signals. There was almost complete broadening of the amide signals from C14, N15, and T16/A16, and significant broadening (i.e. ~70% attenuation) of loop 1 residues. By contrast, E7A showed significantly less broadening. [...] | Simonsen SM et al. (2008) J Biol Chem 283:9805-9813 |
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| Temperature | |
| Acid Hydrolysis | | G6A and E7A showed no degradation at 37 °C for up to 24 h. At 80 °C, they degraded within 90 min. [...] | Simonsen SM et al. (2008) J Biol Chem 283:9805-9813 |
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| Enzymatic Digest | | G6A and E7A mutants of kalata B1 were completely impervious to enzymatic digestion by the three endoproteinases trypsin, chymotrypsin, and proteinase K, as was the prototypic cyclotide kalata B1. [...] | Simonsen SM et al. (2008) J Biol Chem 283:9805-9813 |
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| Nematocidal | | The cyclotides caused a concentration-dependent response in the LDA with the two nematode species. [...] | Colgrave ML et al. (2008) Biochemistry 47:5581-5589 |
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