(185) Assay Card

General Information
Summary Values of minimal inhibitory concentration (MIC) varv peptide B[del8] on different Gram-negative bacterial fish pathogens. F. psychrophilum was the bacteria most susceptible, with an MIC of 12.5 +/- 0.3 uM.
Condition A range of peptide concentrations (1–50 uM) were mixed with 100 uL of an exponential phase bacterial culture of Vibrio anguillarum, Vibrio ordalii, Flavobacterium psychrophilum, Aeromonas hydrophila, and Aeromonas salmonicida. A phospholipase-A2-derived synthetic peptide variant was used as a positive control.
Result MIC on F. psychrophilum, A. hydrophila, A. salmonicida, V. anguillarum, and V. ordalii are 12.5 +/- 0.3 uM, 22.5 +/- 0.3 uM, 22.5 +/- 0.7 uM, 30 +/- 1.2 uM, and 30 +/- 0.8 uM, respectively.
AssayType Anti-bacterial

Álvarez, C.A., Santana, P.A., Luna, O., Cárdenas, C., Albericio, F., Romero, M.S., and Guzmán, F. (2018) Chemical Synthesis and Functional Analysis of VarvA Cyclotide Molecules 23:952-0

Proteins Assayed varv peptide B[del8]