(119) Assay Card

General Information
Summary Cyclotides show molluscicidal activity
Condition Solutions of the crude cyclotide extracts from O. affinis and V. odorata were made up to 145, 290, 435, and 580 µg/mL in distilled water. Ten snails were placed in 200 mL glass beakers containing 50 mL each of the cyclotide solutions and incubated for 24 h at room temperature (28 2 °C). During this period, a plastic mesh was fitted into the beakers to prevent the snails from moving above the level of the solution. The snails were not fed. After this time, the snails were removed from the solutions, washed 10 times in water, and then placed in 50 mL of water for a further 24 h to recover. During the recovery period, the snails were provided with rolled oats (~50 mg). Mortality rates were determined at the end of the recovery period by observing the heartbeat (which can be seen through the shell) and/or the retraction reflex of the foot of the snail after being pulled gently. The experiment was repeated four times at each concentration.
Result Kalata B1, kalata B2, and cycloviolacin O1 displayed an average mortality of 68%, 78%, and 100%, respectively, which was approximately equal to or higher than metaldehyde at 60%. In contrast, kalata B7 and kalata B8 had little effect on snail mortality.
AssayType Molluscicidal

Plan MR, Saska I, Cagauan AG, Craik DJ (2008) Backbone cyclised peptides from plants show molluscicidal activity against the rice pest Pomacea canaliculata (golden apple snail) J Agric Food Chem 56:5237-5241

Proteins Assayed kalata B1
cycloviolacin O1
kalata B8
kalata B2
kalata B7