(110) Assay Card

General Information
Summary In the presence of Mn2+ kalata B1, T16A and T27A showed specific broadening of a defined set of amide signals. There was almost complete broadening of the amide signals from C14, N15, and T16/A16, and significant broadening (i.e. ~70% attenuation) of loop 1 residues. By contrast, E7A showed significantly less broadening.
Condition Monitored by NMR.
Result In the presence of Mn2+ kalata B1, T16A and T27A showed specific broadening of a defined set of amide signals. There was almost complete broadening of the amide signals from C14, N15, and T16/A16, and significant broadening (i.e. ~70% attenuation) of loop 1 residues. By contrast, E7A showed significantly less broadening.
AssayType Metal binding assay

References
Simonsen SM, Sando L, Rosengren KJ, Wang CK, Colgrave ML, Daly NL, Craik DJ (2008) Alanine scanning mutagenesis of the prototypic cyclotide reveals a cluster of residues essential for bioactivity J Biol Chem 283:9805-9813

Cross-references
Proteins Assayed kalata B1
[E7A]kalata B1
[T16A]kalata B1
[T27A]kalata B1