(106) Assay Card

General Information

Summary	Substitution of certain residues of kalata B1 to alanine result in loss of insecticidal activity
Condition	Insecticidal activity of the alanine mutants was assessed by measuring their impact on the development of first and second instars of Drosophila melanogaster to adulthood. This assay consumed ~3 mg per test compound by using triplicates at two peptide concentrations. Triplicates were found to vary by <10%.
Result	Relative to 100% control of wild-type peptide at 230 ppm (80 uM) peptide (LD50 = 150 ppm = 52 uM). kB1 (100%), G1A (102%), L2A (100%), P3A (31%), V4A (115%), G6A (2%), E7A (0%), T8A (15%), V10A (108%), G11A (91%), G12A (31%), T13A (118%), N15A (68%), T16A (55%), P17A (83%), G18A (83%), T20A (131%), S22A (118%), V25A (133%), T27A (119%), R28A (100%), N29A (102%)
AssayType	Insecticidal

References

Simonsen SM, Sando L, Rosengren KJ, Wang CK, Colgrave ML, Daly NL, Craik DJ (2008) Alanine scanning mutagenesis of the prototypic cyclotide reveals a cluster of residues essential for bioactivity J Biol Chem 283:9805-9813

Cross-references

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The database and computational tools found on this website may be used for academic research only, provided that it is referred to CyBase, the database of cyclic proteins (http://www.cybase.org.au). For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
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Conan Wang
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Last updated: Saturday 23 September 2023

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(106) Assay Card